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Long noncoding RNA NBAT1 negatively modulates growth and metastasis of osteosarcoma cells through suppression of

Cheng Yang1, Guijiang Wang1, Jian Yang1

  • 1No.4 Department of Orthopedics, Cangzhou Central HospitalCangzhou 061001, Hebei Province, China.

American Journal of Cancer Research
|November 10, 2017
PubMed

Insights

Long noncoding RNA NBAT1 is downregulated in osteosarcoma (OS) and suppresses tumor growth and metastasis. NBAT1 interacts with miR-21, offering a potential new therapeutic target for this common bone cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma (OS) is the most common primary bone cancer.
  • Long noncoding RNAs (lncRNAs) are crucial regulators in biological processes.
  • NBAT1, a lncRNA, acts as a tumor suppressor in some cancers, but its role in OS is unclear.

Purpose of the Study:

  • To investigate the expression, function, and mechanism of lncRNA NBAT1 in osteosarcoma.
  • To determine if NBAT1 acts as a tumor suppressor in OS.
  • To explore NBAT1's interaction with microRNAs in OS progression.

Main Methods:

  • Analysis of NBAT1 expression in OS tissues and cell lines.
  • Loss- and gain-of-function assays (in vitro and in vivo) to assess NBAT1's role in OS growth and metastasis.
  • Investigation of NBAT1's interaction with miR-21 and its downstream targets.

Main Results:

  • NBAT1 expression is significantly downregulated in OS tissues and cell lines.
  • Downregulated NBAT1 correlates with advanced clinical stage, distant metastasis, and poor prognosis.
  • NBAT1 inhibits OS cell growth and metastasis by suppressing miR-21 and its downstream targets (PTEN, PDCD4, TPM1, RECK).

Conclusions:

  • NBAT1 functions as a tumor suppressor in osteosarcoma.
  • NBAT1 inhibits OS progression by regulating the miR-21 pathway.
  • NBAT1 represents a potential novel therapeutic target for osteosarcoma treatment.
Keywords:
NBAT1growthmetastasismiR-21

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