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Updated: Feb 19, 2026

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
Published on: August 17, 2017
Synthetic Biology to Engineer Bacteriophage Genomes
Ana Rita Costa1, Catarina Milho1, Joana Azeredo1
1LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4700-057, Braga, Portugal.
Abstract:
Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized bacteriophages with new functionalities. Bacteriophages have been engineered towards a wide range of applications including pathogen control and detection, targeted drug delivery, or even assembly of new materials.In this chapter, two strategies that have been successfully used to genetically engineer bacteriophage genomes are addressed: a yeast-based platform and bacteriophage recombineering of electroporated DNA.
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