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Updated: Feb 19, 2026
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Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
Published on: November 5, 2020
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Pancreatic cyst fluid harbors a unique microbiome
Shan Li1, Gwenny M Fuhler1, Nahush Bn2
1Department of Gastroenterology and Hepatology, Erasmus MC - University Medical Center Rotterdam, 's Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands.
Microbiome
|November 11, 2017
Summary
This study found unique bacterial communities in pancreatic cyst fluids, with specific genera like Bacteroides and Escherichia/Shigella being abundant. This pancreatic microbiome may play a role in cancer development.
Area of Science:
- Microbiology
- Gastroenterology
- Oncology
Background:
- Specific intestinal bacteria are linked to gastrointestinal premalignant conditions.
- The microbial composition of pancreatic cystic lesions has not been previously analyzed.
- This study investigates bacterial presence in pancreatic cyst fluids (PCF).
Purpose of the Study:
- To determine if pancreatic cyst fluids (PCF) contain bacterial DNA.
- To identify the bacterial genera present in PCF.
- To characterize the pancreatic cyst fluid microbiome.
Main Methods:
- DNA isolation from 69 PCF samples.
- Bacterial 16S rRNA gene sequencing using conventional PCR and next-generation sequencing (NGS).
- Analysis of the V3-V4 variable region of the 16S rRNA gene.
Main Results:
- 98.2% of PCF samples were PCR-positive for bacteria; 100% were NGS-positive.
- 408 bacterial genera were identified in PCF.
- Bacteroides, Escherichia/Shigella, and Acidaminococcus were predominant genera, with notable Staphylococcus and Fusobacterium.
Conclusions:
- A distinct bacterial ecosystem exists in pancreatic cyst fluid, potentially reflecting the pancreatic local microbiota.
- The identified pancreatic microbiome may contribute to pancreatic neoplastic processes.
- Further research is needed to understand the host-microbiota relationship in pancreatic diseases.
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