Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Feb 19, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

1.1K

The Pediatric Ependymoma Protein Database (PEPD).

George Th Tsangaris1, Athanasios K Anagnostopoulos1

  • 1Proteomics Research Unit, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.

Data in Brief
|November 11, 2017
PubMed
Summary

Researchers analyzed pediatric ependymoma tissue proteomes using mass spectrometry. This study established the Pediatric Ependymoma Protein Database (PEPD), aiding future research into this rare childhood brain tumor.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of a Greek Yoghurt-based Complex on the Human Facial Skin Microbiome.

In vivo (Athens, Greece)·2026
Same author

Optimization of Pyrazolo[3,4‑<i>b</i>]pyridine Analogues: Synthesis, In Vitro Anticancer Evaluation and In Vivo Pharmacokinetics.

ACS omega·2026
Same author

Pregnancy-Associated Glycoproteins Identification in Skopelos Goat Milk by Means of Mass Spectrometry.

Veterinary sciences·2025
Same author

Appraisal of the Use of Proteomics Methodological Approaches and Technologies on Sheep and Goat Research and Clinical Work.

Animals : an open access journal from MDPI·2025
Same author

Detection of Cathelicidin-1 and Cathelicidin-2 Biomolecules in the Milk of Goats and Their Use as Biomarkers for the Diagnosis of Mastitis.

Animals : an open access journal from MDPI·2025
Same author

Propolis Extract with Activity Against <i>Cutibacterium acnes</i> Biofilm Targeting the Expression of Virulence Genes.

Antioxidants (Basel, Switzerland)·2025

Area of Science:

  • Oncology
  • Proteomics
  • Pediatric Medicine

Background:

  • Pediatric brain tumors, including ependymoma, are challenging to study due to sample rarity and collection difficulties.
  • Ependymoma is the third most common pediatric tumor, with largely unknown biological characteristics and reliance on clinical/histological factors for prognosis.
  • Modern holistic technologies like proteomics are underutilized in pediatric brain tumor research.

Purpose of the Study:

  • To perform the first in-depth proteomic analysis of pediatric ependymoma tissue.
  • To establish a high-confidence protein database for pediatric ependymoma.
  • To provide a foundation for targeted protein validation in pediatric ependymoma.

Main Methods:

  • Utilized a modified nano liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) approach for proteomic analysis.
Keywords:
EpendymomaEpendymoma protein databasePediatric brain tumors

More Related Videos

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma DIPG
08:46

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma DIPG

Published on: March 7, 2017

17.6K
Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

909

Related Experiment Videos

Last Updated: Feb 19, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

1.1K
A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma DIPG
08:46

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma DIPG

Published on: March 7, 2017

17.6K
Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

909
  • Applied high-end mass spectrometry for in-depth examination of cancer tissue protein composition.
  • Developed a high-confidence protein database from identified peptides.

    • Main Results:

    • Successfully performed an in-depth proteomic examination of pediatric ependymoma tissue.
    • Constructed the Pediatric Ependymoma Protein Database (PEPD).
    • The PEPD contains 4,157 protein groups derived from over 15,000 identified peptides.

    Conclusions:

    • This study presents the first comprehensive proteomic dataset for pediatric ependymoma.
    • The Pediatric Ependymoma Protein Database (PEPD) is a valuable resource for the scientific community.
    • PEPD serves as a crucial first step toward protein validation and understanding pediatric ependymoma.