Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Coagulation01:06

Coagulation

1.5K
Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
1.5K
Coagulation01:09

Coagulation

11.0K
The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
11.0K
Introduction to Hemostasis01:05

Introduction to Hemostasis

14.9K
Hemostasis is a complex physiological process that prevents excessive bleeding when a blood vessel is injured. It's crucial for maintaining the integrity of the circulatory system, as it ensures that our blood remains fluid while still within the vascular network and yet clots to prevent blood loss upon vessel injury.
The three phases of hemostasis involve many clotting factors present in plasma and several substances released by platelets and injured tissue cells. It is a fast, localized,...
14.9K
Blood Transfusion and Agglutination02:45

Blood Transfusion and Agglutination

15.0K
Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
15.0K
Coulometry: Overview01:00

Coulometry: Overview

2.4K
Coulometry is one of the rapid, most accurate, and precise analytical techniques that determine the quantity of an analyte by measuring the electrical charge needed for its complete electrolysis without using any analytical standards. The total charge passed during electrolysis correlates with the analyte amount by Faraday's laws of electrolysis. For accurate coulometric measurements, a charge equal to Faraday's constant multiplied by the number of electrons involved in the relevant...
2.4K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.3K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alpha-2 antiplasmin deficiency: a rare fibrinolytic disorder identified after decades of diagnostic delay.

Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis·2026
Same author

A Diagnostic Journey through a Rare Hemostatic Disorder.

The journal of applied laboratory medicine·2026
Same author

Should Prediabetes Be Classified as a Treatable Disease?

Journal of clinical medicine·2026
Same author

Simulated Microgravity Causes Delayed Platelet Activation and Downregulates Acid-Sensing Ion Channel 1/2 Protein Expression.

Biomedicines·2025
Same author

Commentary on Blood Cell Agglutination Interference in Complete Blood Count Analysis.

Clinical chemistry·2025
Same author

ADLM Guidance Document on Coagulation Testing in Patients Using Direct Oral Anticoagulants.

The journal of applied laboratory medicine·2025
Same journal

An evaluation of the necessity of repeat testing of critical and dangerous values in a medical center in Taiwan.

Laboratory medicine·2026
Same journal

Epitope analysis aids in identification of donor-specific antibody against HLA-DP29 during virtual crossmatch.

Laboratory medicine·2026
Same journal

Using blood cell histogram analysis for swift anemia diagnosis alongside complete blood cell count and peripheral blood film.

Laboratory medicine·2026
Same journal

Medical laboratory science course performance and grade point average as indicators of ASCP certification success: a 10-year review from an NAACLS-accredited program.

Laboratory medicine·2026
Same journal

Renal magnesium wasting and functional hypoparathyroidism: a diagnostic and therapeutic challenge in hypocalcemia.

Laboratory medicine·2026
Same journal

Variable performance of 5 detection methods for identifying intravenous fluid contamination in basic metabolic panels at an academic medical center.

Laboratory medicine·2026
See all related articles

Related Experiment Video

Updated: Feb 19, 2026

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization
06:28

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Published on: June 4, 2020

7.3K

Coagulation Testing in the Core Laboratory.

William E Winter1, Sherri D Flax1, Neil S Harris1

  • 1Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, FL.

Laboratory Medicine
|November 11, 2017
PubMed
Summary
This summary is machine-generated.

This study details the intricate processes of primary and secondary hemostasis, including the roles of key factors and diagnostic tests like PT and aPTT. It also covers factor inhibitors, VWF analysis, antiphospholipid syndrome, and direct-acting oral anticoagulants.

Keywords:
coagulationextrinsic pathwayintrinsic pathwaypartial thromboplastin timeprothrombin timethromboembolismvon Willebrand factor

More Related Videos

Author Spotlight: Deciphering Coagulation Disorders in Traumatic Brain Injury Patients
04:56

Author Spotlight: Deciphering Coagulation Disorders in Traumatic Brain Injury Patients

Published on: August 4, 2023

1.2K
In Vitro Thrombosis Test for Ventricular Assist Devices
09:15

In Vitro Thrombosis Test for Ventricular Assist Devices

Published on: March 21, 2025

1.3K

Related Experiment Videos

Last Updated: Feb 19, 2026

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization
06:28

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Published on: June 4, 2020

7.3K
Author Spotlight: Deciphering Coagulation Disorders in Traumatic Brain Injury Patients
04:56

Author Spotlight: Deciphering Coagulation Disorders in Traumatic Brain Injury Patients

Published on: August 4, 2023

1.2K
In Vitro Thrombosis Test for Ventricular Assist Devices
09:15

In Vitro Thrombosis Test for Ventricular Assist Devices

Published on: March 21, 2025

1.3K

Area of Science:

  • Hematology
  • Coagulation Science
  • Clinical Pathology

Background:

  • Primary hemostasis involves endothelial injury, VWF-platelet binding, and aggregation via ADP and thromboxane.
  • Secondary hemostasis is initiated by factor VII and tissue factor, involving vitamin K-dependent factors.

Purpose of the Study:

  • To elucidate the mechanisms of primary and secondary hemostasis.
  • To describe common laboratory tests for coagulation assessment.
  • To discuss specific coagulation disorders and newer anticoagulant therapies.

Main Methods:

  • Review of established knowledge on hemostasis pathways (intrinsic, extrinsic, common).
  • Description of diagnostic assays: PT, aPTT, Clauss assay, mixing studies, factor activity assays.
  • Explanation of VWF multimer analysis and antiphospholipid syndrome diagnostics.

Main Results:

  • Standard coagulation tests (PT, aPTT) have specific applications and limitations.
  • Factor inhibitors and VWF abnormalities require specialized diagnostic approaches.
  • Antiphospholipid syndrome presents with paradoxical thrombotic risk despite prolonged aPTT.
  • Direct-acting oral anticoagulants (DOACs) monitoring remains challenging with standard tests.

Conclusions:

  • Understanding hemostasis pathways and diagnostic tests is crucial for clinical management.
  • Specialized testing is necessary for diagnosing complex coagulation disorders.
  • Current standard coagulation tests are insufficient for monitoring DOACs.