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A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
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High throughput human plasma N-glycan analysis using DNA analyzer and multivariate analysis for biomarker discovery
Hua-Tao Feng1, Siying Lim2, Anna Karen Carrasco Laserna2
1Chemistry Department, National University of Singapore, 3 Science Drive 3, S117543, Singapore; NUS Environmental Research Institute, National University of Singapore, T-Lab Building, 5A Engineering Drive 1, 117411, Singapore.
Analytica Chimica Acta
|November 12, 2017
Summary
High-throughput glycan profiling of human plasma N-glycans shows potential for diagnosing chronic kidney disease and predicting anti-platelet drug response. This method enables simultaneous screening of multiple glycan biomarkers from a single blood drop.
Area of Science:
- Biochemistry and Clinical Diagnostics
- Glycomics and Biomarker Discovery
Background:
- Carbohydrates, particularly N-linked glycans (N-glycans) on proteins, significantly influence biological activities.
- Glycan profiling holds promise for clinical applications due to its link to various bioactivities and disease states.
Purpose of the Study:
- To demonstrate a high-throughput platform for analyzing major N-glycans in human plasma.
- To apply this platform in biomarker studies for chronic kidney disease and anti-platelet drug response in heart disease patients.
Main Methods:
- Utilized GlycanAssure™ sample preparation technology and a multi-capillary DNA analyzer for N-glycan analysis.
- Employed bio-statistical methods, including Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA), for data processing and model building.
- Analyzed 37 N-glycan peaks with confirmed structures for most.
Main Results:
- Achieved high classification accuracy: 95.45% for chronic kidney disease and 85.42% for anti-platelet drug response.
- Demonstrated the platform's ability to differentiate patient groups based on N-glycan profiles.
Conclusions:
- The high-throughput glycan analysis platform is effective for N-glycan profiling in human plasma.
- This platform shows significant potential for simultaneous screening of multiple glycan biomarkers for disease diagnosis and drug response prediction using minimal blood samples.
