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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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Emergency Undocking in Robotic Surgery: A Simulation Curriculum
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Curriculum Using the In-Situ Operating Room Setting.

Raghavendra Rao1, Robert C Caskey1, Lily Owei1

  • 1Department of Surgery, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania.

Journal of Surgical Education
|November 12, 2017
PubMed
Summary
This summary is machine-generated.

Implementing team-based surgical skills training in an operating room setting is feasible and effective. This pilot study demonstrated improved team performance and positive participant feedback, highlighting the benefits of in-situ simulation for general surgery residency programs.

Keywords:
APDS curriculumNOTECHs IIPractice-Based Learning and ImprovementSystems-Based Practicein-situ simulationinterpersonal skillsprofessionalism

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Area of Science:

  • Medical Education
  • Surgical Simulation
  • Team-Based Learning

Background:

  • The American College of Surgeons/Association of Program Directors in Surgery curriculum includes a team-based skills phase (Phase 3) that has seen limited implementation.
  • Simulation-based training is crucial for developing surgical competencies, but implementing advanced phases can be challenging.
  • In-situ simulation offers a potential solution to logistical hurdles in training.

Purpose of the Study:

  • To assess the feasibility of implementing the Phase 3 team-based skills curriculum in an in-situ operating room (OR) setting.
  • To evaluate the effectiveness of in-situ simulation on surgical team performance.
  • To gather participant feedback on the training experience.

Main Methods:

  • Conducted 8 in-situ simulation sessions in an OR at a tertiary care university hospital.
  • Included 8 surgery residents, 16 anesthesia residents, 16 nurses, and 13 ancillary staff.
  • Utilized 14 diverse scenarios (e.g., laparoscopic crisis, postoperative complications) and assessed team performance using the NOTECHs II scale, with audiovisual recording and inter-rater reliability analysis.

Main Results:

  • Significant improvement in total team NOTECHs II scores from the first to the second simulation (69.4 to 77.3, p=0.007).
  • All subteams (surgery, anesthesia, nursing) showed improved NOTECHs II scores.
  • Participant feedback was predominantly favorable, though inter-rater reliability for the NOTECHs II scale was modest.

Conclusions:

  • The in-situ OR environment is a viable and effective setting for team-based surgical training, overcoming logistical challenges.
  • Administrative and departmental buy-in, along with protected faculty time, are essential for successful in-situ training.
  • The NOTECHs II scale is a valid tool for evaluating teams in this setting, but further refinement of inter-rater reliability is needed.