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Updated: Feb 19, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
Published on: August 17, 2015
Ultra-Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal
Lian Zhou1, Shuang Sun1, Wei Zhang1
1State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Nr 800 Dongchuan Rd., Shanghai, 200240, China.
Abstract:
Molecules of the diffusible signal factor (DSF) family represent a class of widely conserved quorum-sensing signals used by many Gram-negative bacterial pathogens. The measurement of DSF family signals is essential for understanding their biological roles, signaling pathway, and regulatory network. We are presenting here methods for extraction and purification of DSF family signals from culture supernatants, and further quantification of members of DSF family signals using an ultra-performance liquid chromatographic system (UPLC) coupled with an accurate time-of-flight mass spectrometry (TOF-MS) analysis.
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