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Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

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Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
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Bacterial Signaling01:30

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Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
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Methods to Study Solo/Orphan Quorum-Sensing Receptors.

Vittorio Venturi1, Sujatha Subramoni2, Anice Sabag-Daigle3

  • 1International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149, Trieste, Italy. venturi@icgeb.org.

Methods in Molecular Biology (Clifton, N.J.)
|November 14, 2017
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Summary
This summary is machine-generated.

LuxR solos/orphans are widespread bacterial receptors crucial for cell-cell communication. This study introduces three methods to investigate these understudied quorum sensing regulators.

Keywords:
E. coliLuxILuxR orphansLuxR solosQuorum sensingRegulonSalmonellaSdiATranscriptional fusionTransposon

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Area of Science:

  • Microbiology
  • Bacterial communication
  • Molecular biology

Background:

  • LuxR solos/orphans are a widespread class of transcriptional regulators in Proteobacteria.
  • Despite their prevalence, these receptors are significantly understudied in the context of bacterial cell-cell communication.
  • Quorum sensing (QS) is a critical process for bacterial coordination, and LuxR solos/orphans are implicated in this pathway.

Purpose of the Study:

  • To address the understudied nature of LuxR solos/orphans.
  • To provide accessible methodologies for researchers to begin studying this receptor subgroup.
  • To facilitate further investigation into the role of LuxR solos/orphans in bacterial quorum sensing.

Main Methods:

  • Description of three distinct and simple experimental approaches.
  • Focus on methodologies applicable to studying LuxR solos/orphans.
  • Guidance for initiating research on these specific quorum sensing receptors.

Main Results:

  • The abstract does not contain specific results, but outlines the introduction of new study methodologies.
  • The described methods are designed to be practical for initial investigations.
  • These approaches aim to enable the study of LuxR solos/orphans' function.

Conclusions:

  • LuxR solos/orphans represent an important, yet neglected, area of quorum sensing research.
  • The presented methodologies offer a starting point for exploring their roles in bacterial communication.
  • Further research into LuxR solos/orphans is warranted to understand their contribution to bacterial systems.