A Culture-Dependent Method for the Identification of Quorum Quenching Enzymes of Microbial Origin

Although it has been more than a decade since the first discovery of AHL lactonase AiiA in Bacillus sp. 240B1, we are only beginning to understand the diversity of quorum quenching (QQ) enzymes. Most of the previously identified QQ enzymes are derived from nonmarine microorganisms. A novel marine-derived secretory AHL lactonase, MomL, was found in Muricauda olearia in our previous work and represents a novel type of AHL lactonase widespread in the ocean. Herein, we describe a culture-dependent method for the identification of microbial QQ enzymes, especially the high-throughput method for screening QQ bacteria from cultivable bacterial strains. This method should be capable of efficiently identifying QQ enzymes from various microbial origins. The discovery of more QQ enzymes will help us to understand their ecological roles and may provide potential as therapeutic agents.