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Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures
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In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes
Jasmine Chamiolo1, Imre Gaspar2, Anne Ephrussi2
1Department of Chemistry, Humboldt University Berlin, Brook-Taylor-Str. 2, D-12489, Berlin, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|November 14, 2017
Abstract:
Fluorogenic hybridization methods, such as the use of FIT probes, enable the in vivo detection of specific mRNAs transcribed from their endogenous, genetically nonmodified loci. Here, we describe the design, synthesis and injection of nuclease resistant FIT probes into developing Drosophila oocytes to detect endogenous localizing mRNAs as wells as to probe function of structural RNA elements.
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