Development of ¹¹C-Labeled ω-sulfhydryl fatty acid tracer for myocardial imaging with PET

[¹¹C]-S-methyl-16-thiopalmitic acid (a) was developed with excellent heart-to-background uptake ratios and higher retention in heart. Myocardial uptake and metabolism of the tracer is markedly higher CPT I dependent. When compared to [¹¹C]-S-methyl-14-thiomyristic acid (b), [¹¹C]-S-methyl-12-thiododecanoic acid (c) and [¹¹C]-palmitate, a showed an early high uptake and a significantly slower late clearance in heart and a prolonged myocardial elimination half-life (30 min). Analysis of heart tissue and urine samples showed that a was metabolized via beta-oxidation in myocardium. Small animal PET images of the accumulation of a in the rat myocardium were clearly superior to [¹¹C]-palmitate. These initial studies suggest that a could be a potentially useful clinical PET tracer to assess myocardial fatty acid metabolism.