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Updated: Feb 18, 2026

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
Published on: November 7, 2018
Identification of nonserotypeable Shigella spp. using genome sequencing: a step forward
Muthuirulandi Sethuvel Dhiviya Prabaa1,1, Devanga Ragupathi Naveen Kumar1,1, Inbanathan Francis Yesurajan1,1
1Department of Clinical Microbiology, Christian Medical College, Vellore - 632 004, India.
Aim:
Sequencing technology has replaced conventional methods in identifying and characterizing bacterial pathogens. We characterized 23 nonserotypeable Shigella that biochemically resembled Shigella spp. using whole genome sequencing.
Materials & Methods:
Genome sequences were analyzed using online tools based on 16S rRNA, k-mer, gyrB sequences and analysis of O-antigen arrangement was done using PATRIC database for species identification. Sequence types, plasmid types, antimicrobial resistance and virulence genes were also investigated.
Results:
The SpeciesFinder using 16S rRNA sequences identified only 74% of the isolates, whereas KmerFinder and gyrB sequence analysis identified 100% of the isolates to its species level. Antimicrobial resistance, virulence and plasmid incompatibility groups were identified in all the isolates. Sequence types were determined.
Conclusion:
This study shows that whole genome sequencing approach for Shigella O-antigen analysis has greater discriminative power than other methods using different bioinformatics pipeline for identification of nonserotypeable Shigella.
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