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Updated: Feb 18, 2026

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi
Published on: September 28, 2022
Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of
Saija Haapa-Paananen1, Harri Savilahti2
1Division of Genetics and Physiology, Department of Biology, University of Turku, Turku, FI-20014, Finland.
Abstract:
The capacity of transposable elements to insert into the genomes has been harnessed during the past decades to various in vitro and in vivo applications. This chapter describes in detail the general protocols and principles applicable for the Mu in vitro transposition reaction as well as the assembly of DNA transposition complexes that can be electroporated into bacterial cells to accomplish efficient gene delivery. These techniques with their modifications potentiate various gene and genome modification applications, which are discussed briefly here, and the reader is referred to the original publications for further details.
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