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Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
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miR‑660‑5p is associated with cell migration, invasion, proliferation and apoptosis in renal cell carcinoma

Tao He1, Peijie Chen1, Lu Jin1

  • 1Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.

Molecular Medicine Reports
|November 16, 2017
PubMed

Insights

MicroRNA (miR)-660-5p is downregulated in renal cell carcinoma (RCC). Restoring miR-660-5p suppresses RCC cell growth and invasion, suggesting it acts as a tumor suppressor in kidney cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Renal cell carcinoma (RCC) is a prevalent urinary system malignancy with a poor prognosis.
  • MicroRNAs (miRNAs) are small, non-coding RNAs crucial in tumor development.
  • The specific role of miR-660-5p in RCC remains largely undetermined.

Purpose of the Study:

  • To investigate the expression levels of miR-660-5p in RCC tissues.
  • To elucidate the functional role of miR-660-5p in RCC cell proliferation, migration, invasion, and apoptosis.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for miRNA expression analysis.
  • In vitro assays including wound-healing, Matrigel invasion, MTT, Cell Counting Kit-8, and flow cytometry.
  • Manipulation of miR-660-5p levels using synthetic mimics and inhibitors.

Main Results:

  • miR-660-5p expression was significantly downregulated in RCC tissues compared to adjacent normal tissues.
  • Upregulation of miR-660-5p suppressed RCC cell migration, invasion, and proliferation.
  • Restoration of miR-660-5p induced apoptosis in RCC cells, while inhibition promoted these processes.

Conclusions:

  • miR-660-5p exhibits significantly reduced expression in renal cell carcinoma.
  • miR-660-5p functions as a tumor suppressor by inhibiting proliferation and metastasis and promoting apoptosis in RCC.
  • Targeting miR-660-5p may offer a potential therapeutic strategy for renal cell carcinoma.

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