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Three-Dimensional, Enzyme Biohydrogel Electrode for Improved Bioelectrocatalysis.

Ananta Ghimire1, Ajith Pattammattel1, Charles E Maher1

  • 1Department of Chemistry, University of Connecticut , Storrs, Connecticut 06269-3060, United States.

ACS Applied Materials & Interfaces
|November 16, 2017
PubMed
Summary
This summary is machine-generated.

Biohydrogels significantly boost enzyme loading and electron transfer for biofuel cells. This advancement enhances current density and enzyme stability, paving the way for improved bioelectronic devices.

Keywords:
EDC couplingbioelectrocatalysisbiofuel cellcarbon clothglucose oxidasehigh current densitymobile mediatorsprotein network

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Area of Science:

  • Bioelectrochemistry
  • Biomaterials Science
  • Enzyme Engineering

Background:

  • Enhancing current density in biofuel cells requires higher enzyme loading and efficient electron transfer.
  • Current electrode designs limit enzyme loading and can cause enzyme inactivation due to unfavorable surface interactions.

Purpose of the Study:

  • To develop a biohydrogel strategy for increased enzyme loading and stability on electrodes for biofuel cell applications.
  • To investigate the impact of biohydrogels on enzyme electroactivity and electron transfer efficiency.

Main Methods:

  • Enzymes (glucose oxidase) were embedded within a bovine serum albumin biohydrogel matrix on carbon cloth electrodes.
  • The bioelectrode performance was evaluated using ferricyanide as a mediator in the presence of glucose.
  • Key parameters such as current density, sensitivity, half-life, and enzyme electroactivity were measured.

Main Results:

  • The biohydrogel electrode achieved a maximum current density of 13.2 mA·cm⁻² with glucose oxidase.
  • A soluble mediator enhanced current density by approximately 1000-fold.
  • A record 2.2% of loaded enzyme was electroactive, and enzymatic activity was retained across a wide pH range (4.0-8.0).

Conclusions:

  • Biohydrogels provide a 3D matrix that enhances enzyme density and protects against degradation, improving biofuel cell performance.
  • The developed biohydrogel system demonstrates excellent potential for enzymatic electron transfer reactions in bioelectronics and biofuel cells.
  • Optimization of mediators and buffers (citrate-phosphate) is crucial for maximizing current density.