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Assessing Specimens of Devitalized Tissue in Chronic Sacral Pressure Ulcers: A Pilot Study
Juliany Lino Gomes Silva1, Andressa Coope, Eliana Pereira de Araújo
1At the University of Campinas in São Paulo, Brazil, Juliany Lino Gomes Silva, MS, RN, is a PhD Student, Faculty of Nursing; Andressa Coope, PhD, is a Postdoctoral Fellow, Laboratory of Cell Signaling, School of Medical Sciences; Eliana Pereira de Araújo, PhD, RN, is Professor, Faculty of Nursing; Licio A. Velloso, MD, PhD, is Professor, Department of Internal Medicine, School of Medical Sciences; Mário José A. Saad, MD, PhD, is Professor, Department of Internal Medicine, School of Medical Sciences; and Maria Helena Melo Lima, PhD, RN, is Professor, Faculty of Nursing.
This study analyzed devitalized tissue from pressure ulcers (PrUs), finding altered gene expression that promotes inflammation. Understanding these wound inflammation mechanisms may lead to new therapeutic strategies for chronic wounds.
Area of Science:
- Biomedical Science
- Wound Healing Research
- Molecular Biology
Background:
- Pressure ulcers (PrUs) are chronic wounds resulting from factors like ischemia, bacterial colonization, and hypoxia.
- Altered cellular and systemic stress responses also contribute to PrU development.
- Understanding the molecular mechanisms of PrUs is crucial for developing effective treatments.
Purpose of the Study:
- To analyze gene expression in devitalized tissue from Stages III and IV PrUs.
- To compare gene expression in PrU tissue with healthy skin samples.
- To evaluate the role of specific genes in wound inflammation.
Main Methods:
- Sharp debridement was used to collect tissue samples from PrU patients and healthy controls.
- Gene expression analysis was performed on the collected tissue samples.
- Fibroblast cultures were treated with devitalized tissue extract, followed by Western blotting analysis.
Main Results:
- Gene expression analysis revealed 5 up-regulated and 6 down-regulated genes in devitalized PrU tissue.
- Fibroblast cultures exposed to devitalized tissue extract exhibited reduced cell attraction and growth.
- Western blotting indicated a decreasing trend in 3 specific genes in treated cell cultures.
Conclusions:
- The identified mediators in devitalized tissue stimulate and promote inflammation in the wound bed.
- Altered gene expression in PrUs contributes to the inflammatory process.
- Further understanding of chronic wound development mechanisms can inform novel therapeutic strategies.
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