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Profiling membrane glycerolipids during γ-ray-induced membrane injury
Guowei Zheng1,2, Weiqi Li3,4
1Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, People's Republic of China.
BMC Plant Biology
|November 17, 2017
Summary
Gamma rays cause plant cell membrane injury, impacting glycerolipid metabolism. Phospholipase D (PLD) plays a role in this process, with specific lipids degrading differently under radiation.
Area of Science:
- Plant Biology
- Biochemistry
- Radiation Biology
Background:
- Gamma rays induce random plant cell injuries, with limited understanding of their effects on glycerolipid metabolism.
- Previous research focused on nucleotide damage and reactive oxygen species (ROS), neglecting membrane lipid changes.
- Investigating glycerolipid metabolism is crucial for understanding gamma-ray-induced membrane injury in plants.
Purpose of the Study:
- To investigate the role of phospholipase D (PLD)-mediated glycerolipid metabolism in gamma-ray-induced membrane injury.
- To analyze lipidome changes in wild-type and PLD-deficient Arabidopsis under gamma-ray treatment.
- To elucidate the mechanisms of membrane damage caused by high-energy radiation.
Main Methods:
- Utilized an ESI-MS/MS-based lipidomic method for comprehensive lipid analysis.
- Treated wild-type and mutant Arabidopsis (PLDδ- and PLDα1-deficient) with varying doses of gamma rays.
- Measured ion leakage and intracellular ROS accumulation to assess membrane integrity and cellular stress.
Main Results:
- High-dose gamma-ray treatment induced intracellular ROS accumulation but minimal ion leakage (<10%).
- Gamma-ray-induced glycerolipid degradation primarily affected chloroplastidic lipids.
- Specific lysophospholipids (lysoPC, lysoPE) levels varied between ecotypes, while lysophosphatidylglycerol (lysoPG) decreased across genotypes.
Conclusions:
- Gamma-ray-induced membrane injury may involve indirect mechanisms and asynchronous lipid degradation.
- Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) degradation might be mediated by PLDζ1 or phospholipase A1.
- Gamma rays alter membrane lipid properties, decreasing the double-bond index and increasing acyl chain length, potentially increasing membrane rigidity and injury.
