Cytotoxic, Antimitotic, and Antiproliferation Studies on Rasam: A South Indian Traditional Functional Food

Rasam is a traditional South Indian food, prepared using tamarind juice as a base, with a variety of spices. Rasam, with all its ingredients medicinally claimed for various ailments, is a functional food. Systematic consumption of traditional functional food provides an excellent preventive measure to ward off many diseases.

To study rasam for cytotoxic, antimitotic, and antiproliferation potential beyond its culinary and nutritional effect.

Brine shrimp lethality assay, onion root tip inhibition assay, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in Calu-6, HeLa, MCF-7 cell lines for four stage-wise samples in the preparation of rasam (RS1, RS2, RS3, and RS4) were studied.

RS4, the end product of rasam showed high lethality with an LC₅₀ value of 38.7 μL/mL. It showed maximum antimitotic activity in a dose-dependent manner compared to other samples with an IC₅₀ value of 189.86 μL/mL. RS4 also showed an IC₅₀ value of 350.22 and 410.15 μL/mL in MCF-7 and Calu-6 cell lines, respectively.

From this study, we suggest that rasam is a classic example of traditional functional food and it can treat breast and lung cancer on chronic use.

Rasam, a South Indian traditional functional food, showed high lethality (LC₅₀ = 38.7 mL/mL) against brine shrimpsRasam also showed potential antimitotic activity (IC₅₀ = 189.86 mL/mL) by inhibiting the onion root tipsRasam showed an IC₅₀ value of 350.22 and 410.15 mL/mL against MCF-7 and Calu-6 cell lines respectivelyRasam, when consumed on daily dietary basis, can treat breast and lung cancer. Abbreviations used: SS 316: Stainless Steel 316 grade; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMEM: Dulbecco's modified Eagle medium; FBS: Fetal bovine serum media; TPVG: Trypsin phosphate versene glucose; EDTA: Ethylene diamine tetra acetic acid; PBS: Phosphate buffered saline; DMSO: Dimethyl sulfoxide.