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The Membrane Attack Complex/Perforin Superfamily.

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The membrane attack complex/perforin (MACPF) superfamily

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Area of Science:

  • Biochemistry
  • Evolutionary Biology
  • Structural Biology

Background:

  • The membrane attack complex/perforin (MACPF) superfamily is a diverse protein group involved in various biological processes.
  • Previous studies suggested evolutionary relationships based on tertiary structures.

Purpose of the Study:

  • To investigate evolutionary relationships within the MACPF superfamily using primary structure analyses.
  • To identify and classify members of the MACPF superfamily, including MACPF, CDC, and pleurotolysin B families.

Main Methods:

  • Comparative analysis of protein primary structures.
  • Expansion of protein families against a large database.
  • Hidden Markov model comparisons.
  • Clustering analyses.

Main Results:

  • Evolutionary relationships within the MACPF superfamily are supported by primary structure analyses.
  • The superfamily comprises the MACPF, cholesterol-dependent cytolysin (CDC), and pleurotolysin B families.
  • Clustering analyses revealed distinct groupings for CDC, MACPF subfamilies, and pleurotolysin B.
  • MACPF superfamily members are found across eukaryotic, bacterial, and archaeal domains with diverse architectures.

Conclusions:

  • Primary structure analysis provides robust support for evolutionary relationships within the MACPF superfamily.
  • The study clarifies the classification and evolutionary divergence of MACPF, CDC, and pleurotolysin B families.
  • The developed strategy can aid in classifying other divergent protein families.