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Published on: March 11, 2022
Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant
Hiroya Ito1,2,3,4,5, Sayaka Takahashi1,2,3,4,5, Tetsuo Asai1,2,3,4,5
1The National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan (Ito).
Abstract:
An atypical urease-negative mutant of Actinobacillus pleuropneumoniae serovar 2 was isolated in Japan. Nucleotide sequence analysis of the urease gene cluster revealed that the insertion of a short DNA sequence into the cbiM gene was responsible for the urease-negative activity of the mutant. Veterinary diagnostic laboratories should be watchful for the presence of aberrant urease-negative A. pleuropneumoniae isolates.
