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Holistic bioengineering: rewiring central metabolism for enhanced bioproduction
Selçuk Aslan1, Elad Noor2, Arren Bar-Even3
1Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm 14476, Germany.
The Biochemical Journal
|November 18, 2017
Summary
Transforming organisms into biofactories requires optimizing central metabolism, not just production pathways. A holistic bioengineering approach, managing competing pathways and energy resources, is key for efficient bioproduction.
Area of Science:
- Metabolic Engineering
- Synthetic Biology
- Biotechnology
Background:
- Converting organisms into biofactories necessitates optimizing their endogenous metabolic networks.
- Standalone pathway optimization is insufficient; central metabolism must support bioproduction.
Purpose of the Study:
- To review strategies for engineering host metabolic networks for enhanced bioproduction.
- To highlight the importance of a holistic approach in metabolic bioengineering.
Main Methods:
- Exploration of strategies including pathway tuning, precursor availability enhancement, and energy resource management (ATP and NADPH).
- Focus on key metabolic pathways: glycolysis, anaplerosis, TCA cycle, and NADPH production.
Main Results:
- Successful biofactories require modulation of central metabolic pathways.
- Tuning-down competing pathways, increasing precursor supply, and ensuring energy availability are crucial.
Conclusions:
- A holistic bioengineering strategy, considering the entire metabolic network, is essential for creating efficient microbial biofactories.
- Focusing solely on the production pathway limits the potential of biofactories.
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