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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
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NIR Emitting Nanoprobes Based on Cyclic RGD Motif Conjugated PbS Quantum Dots for Integrin-Targeted Optical

N Depalo1, M Corricelli1, I De Paola2

  • 1Istituto per i Processi Chimico-Fisici-CNR SS Bari , Via Orabona 4, 70125 Bari, Italy.

ACS Applied Materials & Interfaces
|November 18, 2017
PubMed
Summary

Researchers developed silica-coated lead sulfide (PbS) quantum dots (QDs) for near-infrared (NIR) imaging. These nanoprobes target tumors by binding to αvβ3 integrins, showing promise for in vivo cancer detection.

Keywords:
NIR emitting quantum dotscyclic RGD peptidesilica-coated nanoprobestargeted imagingαvβ3 integrin receptor

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Materials Science

Background:

  • Hydrophobic lead sulfide (PbS) quantum dots (QDs) exhibit near-infrared (NIR) photoluminescence useful for biological imaging.
  • Improving the aqueous dispersibility of PbS QDs is crucial for in vivo applications.
  • Targeted delivery of nanoprobes to tumors requires specific molecular recognition strategies.

Purpose of the Study:

  • To develop silica-coated PbS QDs as single-particle optical nanoprobes for in vivo tumor imaging.
  • To functionalize these nanoprobes with a cyclic arginine-glycine-aspartic acid (cRGD) peptide for tumor-specific targeting.
  • To evaluate the targeting specificity and imaging potential of the developed nanoprobes.

Main Methods:

  • Silica shell growth on PbS QDs using a base-assisted water-in-oil microemulsion method.
  • Conjugation of silica-coated PbS QDs with cRGD peptide.
  • Characterization using optical and structural techniques.
  • Assessment of cellular uptake and αvβ3 integrin targeting specificity in human melanoma cells via confocal microscopy and cytometry.

Main Results:

  • Successfully synthesized silica-coated PbS QDs with stable NIR photoluminescence in aqueous solutions.
  • Demonstrated specific binding and cellular uptake of cRGD-functionalized nanoprobes in αvβ3 integrin-overexpressing melanoma cells.
  • Confirmed the selectivity of the nanoprobes for αvβ3 integrin receptors.

Conclusions:

  • Silica-coated PbS QDs functionalized with cRGD peptide are effective NIR-emitting nanoprobes.
  • These nanoprobes show significant potential for αvβ3 integrin receptor-targeted in vivo tumor imaging.
  • The developed nanostructures offer a promising platform for advanced optical diagnostics in oncology.