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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea
Insights
Adenoviruses, particularly AdV41, are frequent causes of gastroenteritis in young children. Adenovirus types AdV31 and AdV7 were specifically linked to these infections, showing genetic similarities to Asian serotypes.
Area of Science:
- Pediatric Infectious Diseases
- Virology
- Molecular Epidemiology
Background:
- Adenoviruses are a common cause of gastroenteritis in children.
- Understanding the prevalence and genetic diversity of adenoviruses is crucial for public health interventions.
Purpose of the Study:
- To determine the frequency, genotype distribution, and genetic relatedness of adenoviruses in children under 5 with diarrhea.
- To investigate the association of adenovirus types with clinical findings, age, and seasonality.
Main Methods:
- Stool samples from 180 children with acute gastroenteritis were analyzed using immune chromatographic test (ICT), enzyme immunoassay (EIA), and polymerase chain reaction (PCR).
- Adenovirus types were identified through nucleotide sequence analysis, and phylogenetic analysis was performed using the neighbor-joining method.
Main Results:
- Polymerase chain reaction (PCR) detected adenoviruses in 13.9% of samples, significantly higher than ICT (5%) and EIA (6.1%).
- The predominant genotypes were Adenovirus 41 (AdV41, 64%), followed by AdV40 (24%), AdV31 (8%), and AdV7 (4%).
- Adenovirus gastroenteritis showed no significant variation across age groups, sex, months, or seasons. AdV31 and AdV7 were associated with gastroenteritis and exhibited 80% similarity to Asian serotypes.
Conclusions:
- Adenovirus type 41 is the most prevalent genotype causing gastroenteritis in this pediatric population.
- Adenovirus types 31 and 7 are associated with gastroenteritis and share genetic similarities with Asian adenovirus serotypes.
Abstract:
Background/aim: To determine the frequency, genotype distribution, and genetic relatedness of adenoviruses in children under 5 years old with diarrhoea and to investigate their distribution according to clinical findings, age, months, and seasons. Materials and methods: Stool samples were collected from 180 children with acute gastroenteritis who presented from July 2007 through June 2011 at the Ankara Training and Education Hospital. Stool samples were analysed by immune chromatographic test (ICT), enzyme immunoassay (EIA), and polymerase chain reaction (PCR). All adenovirus types were determined by nucleotide sequence analysis. A phylogenetic tree was constructed by Mega 6.0 using the neighbour-joining method. Results: Five percent of the samples were positive for adenovirus (9/180) by ICT, 6.1% (11/180) by EIA, and 13.9% (25/180) by PCR. Adenovirus gastroenteritis did not show any differences in age group, sex, month, or season. In this study, 16 (64%) of the PCR positive samples were AdV41, 6 (24%) were AdV40, 2 (8%) were AdV31, and 1 (4%) was AdV7, as determined by nucleotide sequencing. Conclusion: AdV31 and AdV7 were associated with gastroenteritis. Adenovirus serotypes showed a similarity of 80% (20/25) and 20% (5/25) with Asian and American serotypes, respectively.
