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Updated: Feb 18, 2026
Antimicrobial Characterization of Advanced Materials for Bioengineering Applications
Published on: August 4, 2018
The Relationship Between Agar Thickness and Antimicrobial Susceptibility Testing
J Nicole Flanagan1, Todd R Steck1
1Department of Biological Sciences, University of North Carolina - Charlotte, 9201 University City Blvd, Charlotte, NC 28223 USA.
Abstract:
Antimicrobial susceptibility testing can be done using solid or liquid-based medium. Solid-based assays are easy and inexpensive; they are limited by not being as quantitative as liquid-based assays. Agar depth can influence the accuracy of plate-based assays and it is assumed the basis of this effect is antimicrobial agent diffusion. We tested this assumption by using ETEST® to quantitate the relationship between agar depth and minimum inhibitory concentration and zone of inhibition.
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