Related Experiment Video
Updated: Feb 18, 2026
11:56
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
Published on: May 4, 2018
13.1K
RNA-Seq Analysis of Antibiotic-Producing Bacillus subtilis SC-8 Reveals a Role for Small Peptides in Controlling PapR
Byung Wook Yang1, In-Cheol Yeo2, Jae Hee Choi1
1Department of Systems Biotechnology, Chung-Ang University, 4726, Seodong-daero, Anseong-si, Gyeonggi-do, 17546, Republic of Korea.
Applied Biochemistry and Biotechnology
|November 21, 2017
Summary
Bacillus cereus peptides, particularly those containing PapR, influence Bacillus subtilis antibiotic production. The study reveals complex regulatory effects on gene expression and antibiotic biosynthesis.
Area of Science:
- Microbiology
- Molecular Biology
- Genomics
Background:
- Bacillus subtilis SC-8 (BSSC8) exhibits limited antimicrobial activity against the Bacillus cereus group.
- PapR, a peptide from B. cereus, was previously hypothesized to stimulate antibiotic production in BSSC8 by activating PlcR.
- Understanding PapR's role is crucial for elucidating interspecies interactions and BSSC8's antibiotic regulation.
Purpose of the Study:
- To investigate the functional role of PapR in BSSC8 antibiotic production.
- To analyze the impact of B. cereus-derived small peptides on BSSC8 global transcription.
- To compare transcriptomic changes induced by wild-type B. cereus (WTBC) peptides versus papR-deleted mutant (MTBC) peptides.
Main Methods:
- Global transcriptomic profiling of BSSC8 using RNA-Sequencing (RNA-Seq).
- Treatment of BSSC8 cultures with small peptides from WTBC and MTBC strains.
- Comparative analysis of gene expression changes in response to different peptide treatments.
Main Results:
- WTBC small peptides led to significantly higher gene upregulation (80.9%) in BSSC8 compared to MTBC peptides.
- Genes involved in glycolysis and the TCA cycle showed altered transcription in response to both peptide types.
- A substantial portion of glycolysis (35.0%) and TCA cycle (60.0%) gene regulation occurred exclusively with WTBC peptides.
- Expression of antibiotic biosynthetic genes in BSSC8 was further suppressed by WTBC small peptides.
Conclusions:
- B. cereus-derived small peptides, especially those containing PapR, significantly modulate BSSC8 global gene expression.
- PapR influences metabolic pathways like glycolysis and the TCA cycle in BSSC8.
- The presence of PapR in B. cereus peptides leads to decreased antibiotic biosynthesis in BSSC8, suggesting a complex regulatory interaction.
More Related Videos
Related Concept Videos
01:28Gene Regulation in Microbial Communities: Quorum Sensing
740
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
740
01:23Stringent Response in E. coli
404
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
404
01:23Types of RNA
73.2K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
73.2K
01:30Bacterial Signaling
41.6K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
41.6K
01:29Translational Regulation
691
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
691
01:29Coordination of Gene Expression Processes in Bacteria
714
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
714