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CD49f+ mammary epithelial cells decrease in milk from dairy cows stressed by overstocking during the dry period.

Mario Baratta1, Silvia Miretti1, Paolo Accornero1

  • 1Department Veterinary Science,University of Turin,Italy,Largo Braccini 2,10095 Grugliasco (TO),Italy.

The Journal of Dairy Research
|November 21, 2017
PubMed
Summary

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Different dry period management in Holstein Friesian cows altered mammary epithelial cell populations. Early lactation showed significant reductions in CD49f+ and cytokeratin 18+ cells, suggesting potential biomarkers for mammary gland function.

Area of Science:

  • Veterinary Science
  • Animal Science
  • Dairy Cattle Research

Background:

  • Mammary epithelial cell populations change during lactation.
  • Dry period management can influence cow health and productivity.
  • Understanding these changes is crucial for dairy cow welfare and milk production.

Purpose of the Study:

  • To investigate the impact of different dry period management strategies on mammary epithelial cell populations in Holstein Friesian cows.
  • To identify specific cell subpopulations (CD49f+ and cytokeratin 18+) that may be affected by management.
  • To explore the potential of these cell phenotypes as biomarkers for mammary gland function.

Main Methods:

  • Twenty-six Holstein Friesian cows were divided into two groups with different stocking densities (11 m2 vs. 5 m2) during the dry period.
Keywords:
biomarkerdairy cowintegrin alpha 6stress

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  • Milk samples were collected during early and late lactation.
  • Flow cytometry was used to analyze cell populations, including CD45, CD49f, cytokeratin 14, and cytokeratin 18 expression, along with cell viability.

    • Main Results:

    • A significant reduction in CD49f+ cells (P < 0.01) and cytokeratin 18+ cells (P < 0.05) was observed in early lactation milk samples from cows managed under different dry period stocking densities.
    • These differences, while less pronounced, were still evident in late lactation.
    • Cell viability did not differ significantly between groups.

    Conclusions:

    • Mammary epithelial cell immunophenotypes, specifically CD49f+ and cytokeratin 18+ subpopulations, are differentially expressed in response to varying dry period management in dairy cows.
    • These findings suggest that these cell types may serve as valuable biomarkers for assessing mammary gland status and function in dairy cattle.
    • Further research is warranted to fully elucidate the role of these biomarkers in dairy cow management and health.