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Related Experiment Video

Updated: Feb 18, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Cell membrane dynamics induction using optogenetic tools.

Yoshibumi Ueda¹, Moritoshi Sato²

¹Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan; AMED-PRIME, Japan Agency for Medical Research and Development, Tokyo, Japan.

Biochemical and Biophysical Research Communications

|November 21, 2017

Summary

This summary is machine-generated.

Optogenetic tools, like the Magnet system, enable precise control over cell membrane dynamics. This technology allows researchers to study actin-regulated cell movements and discover new membrane structures with high spatial and temporal resolution.

Keywords:

Actin Cell membrane Dorsal ruffle Magnet system Optogenetics Photoswitch

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An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

Area of Science:

Cell Biology
Molecular Biology
Biotechnology

Background:

Actin-based cell membrane structures (ruffles, lamellipodia, filopodia) are crucial for cellular functions like motility and neuronal guidance.
Previous methods for studying these dynamics lacked spatial and temporal precision in living organisms.
Optogenetic tools offer a promising alternative for precise modulation of cellular processes.

Purpose of the Study:

To review recent advances in optogenetic tools for inducing actin-regulated cell membrane dynamics.
To introduce the Magnet system, a novel optogenetic tool for precise control over Tiam1 and PIP3 signaling.
To highlight the discovery of unique membrane ruffles using the Magnet system.

Main Methods:

Development and application of the Magnet optogenetic system.
Utilizing Tiam1 and PIP3 signal transduction pathways.
Investigating actin-based cell membrane dynamics with high spatial and temporal resolution.

Main Results:

The Magnet system allows for precise, optogenetic control of cell membrane dynamics.
This system enables the study of Tiam1 and PIP3 signaling in regulating cellular structures.
Unique membrane ruffles were discovered and characterized using this novel optogenetic approach.

Conclusions:

Optogenetic tools, including the Magnet system, provide unprecedented control over cell membrane dynamics.
These tools are valuable for dissecting the molecular mechanisms underlying cellular functions.
The Magnet system facilitates the discovery of novel cellular structures and signaling pathways.