A Red Light-Activatable Endogenous Gene Transcription System with Red-CPTS
Takahiro Nakajima1,2, Yuto Kuwasaki2, Shota Yamamoto2
1Kanagawa Institute of Industrial Science and Technology, Kawasaki-shi, Kanagawa, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|December 26, 2024
Abstract:
Red light penetrates deep into mammalian tissues and has low phototoxicity. We developed a red light-activatable photoswitch (MagRed) for deep tissue optogenetics. Using MagRed, we developed a red light-activatable endogenous gene transcription system (Red-CPTS) based on CRISPR-Cas9. Here we provide a detailed protocol for endogenous gene activation using Red-CPTS in cultured mammalian cells and living mice in vivo.
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