Related Experiment Video
Updated: Feb 18, 2026

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
Published on: April 7, 2023
Optimum Thermal Processing for Extended Shelf-Life (ESL) Milk.
1School of Agriculture and Food Sciences, University of Queensland, Brisbane 4072, Australia. h.deeth@uq.edu.au.
Extended shelf-life (ESL) milk requires intense thermal processing to inactivate bacteria and spores while preserving quality. Achieving over 30 days of refrigerated life balances safety with desirable organoleptic characteristics.
Area of Science:
- Food Science
- Microbiology
- Dairy Technology
Background:
- Extended shelf-life (ESL) milk production involves thermal processing between HTST and UHT methods.
- ESL milk requires a refrigerated shelf-life exceeding 30 days.
- Balancing bacteriological quality, safety, and organoleptic properties is crucial for ESL milk.
Purpose of the Study:
- To inactivate vegetative bacteria and psychrotrophic bacterial spores, particularly *Bacillus cereus*.
- To minimize chemical changes, specifically β-lactoglobulin (β-Lg) denaturation, which causes cooked flavor.
- To establish optimal thermal processing parameters for ESL milk production.
Main Methods:
- Intense thermal processing, focusing on high temperature and short holding times.
- Direct heating methods and aseptic packaging are employed.
- Monitoring bactericidal effect (B*) and β-Lg denaturation levels.
Main Results:
- The study proposes specific targets for thermal processing: B* > 0.3 and ≤50% β-Lg denaturation.
- High temperature, short-time direct heating is identified as optimal.
- Aseptic packaging is essential for maintaining product quality.
Conclusions:
- Optimal thermal processing for ESL milk involves achieving a B* > 0.3 and ≤50% β-Lg denaturation.
- Direct heating and aseptic packaging are key to producing high-quality ESL milk.
- This approach ensures bacteriological safety and desirable sensory attributes.
Related Concept Videos
Physical Methods for Controlling Microbial Growth: Temperature
Factors Influencing Microbial Growth: Temperature
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...

