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A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
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Expression and function of protein A in Staphylococcus pseudintermedius.

Manasi Balachandran1, David A Bemis1, Stephen A Kania1

  • 1a Department of Biomedical and Diagnostic Sciences , Comparative and Experimental Medicine, University of Tennessee , 2407 River Drive, Knoxville , TN , USA.

Virulence
|November 22, 2017
PubMed
Summary
This summary is machine-generated.

Staphylococcus pseudintermedius produces Protein A, a virulence factor that binds canine IgG. Blocking Protein A enhances phagocytosis, suggesting its role in immune evasion during canine pyoderma.

Keywords:
Fc-bindingProtein AStaphylococcus pseudintermediusimmune evasionvaccinevirulence

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Area of Science:

  • Veterinary Microbiology
  • Immunology
  • Bacterial Pathogenesis

Background:

  • Staphylococcus pseudintermedius is a common cause of canine pyoderma.
  • Protein A is a known virulence factor in Staphylococcus aureus, encoded by the spa gene.
  • The presence and function of analogous genes in S. pseudintermedius are not well-defined.

Purpose of the Study:

  • Investigate the presence and expression of spa gene orthologs in S. pseudintermedius.
  • Characterize the protein A produced by S. pseudintermedius.
  • Determine the role of protein A in S. pseudintermedius virulence.

Main Methods:

  • Screening of S. pseudintermedius isolates for spa gene orthologs.
  • Quantification of gene expression using mRNA profiling.
  • Measurement of canine IgG binding to bacterial surface.
  • Assessment of phagocytosis assays with and without protein A blockade.

Main Results:

  • S. pseudintermedius isolates express genes analogous to S. aureus spa.
  • Specific clonal types (ST68, ST71) exhibited higher canine IgG binding, indicating greater surface protein A density.
  • Protein A was secreted during exponential growth and bound IgG via the Fc region.
  • Blocking protein A significantly enhanced phagocytosis by canine immune cells.

Conclusions:

  • S. pseudintermedius produces protein A with immunoglobulin-binding capabilities similar to S. aureus.
  • Protein A functions as a virulence factor by potentially evading the canine host immune system.
  • Targeting protein A could be a strategy to combat S. pseudintermedius infections.