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Updated: Feb 18, 2026

Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method
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Determining Inorganic and Organic Phosphorus.

Jaana Koistinen1, Mervi Sjöblom2, Kristian Spilling3

  • 1Tvärminne Zoological Station, University of Helsinki, Hanko, Finland. jaana.koistinen@helsinki.fi.

Methods in Molecular Biology (Clifton, N.J.)
|November 22, 2017
PubMed
Summary

This study details a colorimetric method for measuring orthophosphate (PO4) and dissolved organic phosphorus (DOP) in microalgal samples. The method also determines particulate organic phosphorus (POP) using total phosphorus measurements.

Keywords:
OrthophosphateParticulate organic phosphorusTotal phosphorus

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Area of Science:

  • Environmental science
  • Biochemistry
  • Analytical chemistry

Background:

  • Phosphorus (P) is an essential macronutrient for microalgal growth.
  • Orthophosphate (PO4) is the primary form of phosphorus utilized by microalgae.
  • Accurate quantification of different phosphorus fractions is crucial for understanding algal nutrient dynamics.

Purpose of the Study:

  • To present a novel colorimetric method for quantifying orthophosphate (PO4) and dissolved organic phosphorus (DOP).
  • To describe a complementary method for determining particulate organic phosphorus (POP).
  • To provide researchers with reliable tools for assessing phosphorus availability in microalgal systems.

Main Methods:

  • A colorimetric assay was developed for the determination of PO4 and DOP concentrations.
  • The method is based on total phosphorus (TP) measurements.
  • A parallel method was established for the quantification of particulate organic phosphorus (POP) using similar principles.

Main Results:

  • The study successfully established a colorimetric technique for measuring PO4 and DOP.
  • The developed method allows for the accurate determination of POP.
  • These methods provide a comprehensive approach to phosphorus fraction analysis in microalgal research.

Conclusions:

  • The presented colorimetric methods offer a robust and efficient means to quantify key phosphorus fractions (PO4, DOP, POP).
  • These techniques are valuable for ecological and biochemical studies involving microalgae.
  • The study contributes to improved understanding of phosphorus cycling in aquatic environments.