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Related Experiment Video

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Transit time mapping in the mouse brain using time-encoded pCASL.

Lydiane Hirschler1,2,3,4, Leon P Munting4,5, Artem Khmelinskii6,7

  • 1Université Grenoble Alpes, Grenoble Institut des Neurosciences, Grenoble, France.

NMR in Biomedicine
|November 22, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces time-encoded pseudo-continuous arterial spin labeling (te-pCASL) for measuring arterial transit time (ATT) in mice. This novel method enables efficient and accurate ATT mapping, crucial for neurological disease research.

Keywords:
ageingarterial spin labelingarterial transit timecerebral blood flowmicetime-encoded pCASL

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Area of Science:

  • Neuroimaging
  • Vascular Biology
  • Biomarker Discovery

Background:

  • Cerebral blood flow (CBF) is a key biomarker for neurological diseases.
  • Arterial transit time (ATT) impacts CBF quantification and may indicate vascular pathologies.
  • Existing magnetic resonance imaging (MRI) methods for ATT measurement in mice are suboptimal.

Purpose of the Study:

  • To adapt and validate time-encoded pseudo-continuous arterial spin labeling (te-pCASL) for measuring ATT in mice.
  • To establish an optimized te-pCASL sequence for high-fidelity ATT mapping in rodent brains.
  • To assess the utility of te-pCASL in detecting vascular changes in mouse models.

Main Methods:

  • Implementation of an 11-sub-bolus, 50 ms time-encoding scheme within pCASL for mice.
  • Acquisition of separate traditional pCASL data for perfusion imaging.
  • Analysis of ATT across six distinct brain regions in healthy and diseased mice.

Main Results:

  • Successful demonstration of te-pCASL for ATT measurement in mice.
  • Identification of regional ATT variations, with the hippocampus showing the shortest ATT (169 ± 11 ms) and the auditory/visual cortex the longest (284 ± 16 ms).
  • Observed preserved ATT in aged wild-type mice and prolonged ATT in a mouse model of carotid artery occlusion.

Conclusions:

  • te-pCASL is a viable and time-efficient method for mouse brain ATT measurement.
  • This technique provides a new tool for studying vascular function and pathology in mice.
  • te-pCASL has the potential to advance research into neurological diseases and aging through improved ATT quantification.