Related Experiment Video
Updated: Feb 18, 2026
Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
Published on: October 25, 2018
Facile immobilization of DNA using an enzymatic his-tag mimic
Pascal Röthlisberger1, Fabienne Levi-Acobas, Ivo Sarac
1Laboratory for Bioorganic Chemistry of Nucleic Acids, Department of Structural Biology and Chemistry, Institut Pasteur, CNRS UMR3523, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France. marcel.hollenstein@pasteur.fr.
Abstract:
Methods for immobilization of DNA on solid supports are in high demand. Herein, we present a generally applicable enzymatic method for the immobilization of DNA without any prior chemical derivatization. This strategy relies on the homopolymerization of the modified triphosphate dImTP by the TdT. The resulting enzymatic his-tag mimic ensures binding of DNA on Ni-NTA agarose. The usefulness of this method is highlighted by the immobilization of functional nucleic acids without impairing their specific activities.
