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Upgrading well plates using open microfluidic patterning.

Samuel B Berry1, Tianzi Zhang, John H Day

  • 1Department of Chemistry, University of Washington, Seattle, Washington 98195, USA. abt1@uw.edu.

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|November 23, 2017
PubMed
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Researchers developed the Monorail Device, an open-microfluidic platform for segregated cell coculture. This system enables direct upgrades of monoculture experiments to multiculture assays, facilitating study of cellular communication.

Area of Science:

  • Cell Biology
  • Microfluidics
  • Biotechnology

Background:

  • Cellular communication is crucial for physiological responses.
  • In vitro coculture systems aim to mimic in vivo environments but face limitations in engineering soluble factor signaling.
  • Current systems struggle to integrate engineering requirements with biocompatible platforms.

Purpose of the Study:

  • To introduce a novel open-microfluidic platform, the Monorail Device, for segregated coculture.
  • To enable seamless upgrading of monoculture experiments to multiculture assays.
  • To facilitate the study of soluble factor signaling in complex cellular systems.

Main Methods:

  • The Monorail Device utilizes microfluidic spontaneous capillary flow (SCF) to pattern biocompatible hydrogel walls.

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  • The platform is designed for use in standard well plates and Petri dishes, creating customized, pipette-accessible chambers.
  • It allows patterning on native cell culture surfaces without modification and creates permeable dividers for factor diffusion.

    • Main Results:

    • The device successfully patterns hydrogel walls in precise shapes on various cell culture surfaces.
    • Reproducible SCF-driven flow is achieved, with characterized physical parameters and specialized design features for ease of use.
    • Hydrogel walls demonstrate integrity and permeability suitable for coculture, supporting surface-sensitive cell culture.

    Conclusions:

    • The Monorail Device offers a versatile and user-friendly solution for segregated coculture.
    • It simplifies the transition from monoculture to multiculture assays, reducing cell requirements.
    • The platform shows potential for modular multikingdom cultures and broad adaptation across research fields.