ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction
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Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Hiba Baaziz1,2, Cyril Gambari1, Anne Boyeldieu1
1Unité de Bioénergétique et Ingénierie des Protéines, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université, Centre National de la Recherche Scientifique, Marseille, France.
Abstract:
The chromate efflux pump encoding gene chrASO was identified on the chromosome of Shewanella oneidensis MR1. Although chrASO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrASO exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrASO in E. coli confers resistance to high chromate concentration. Moreover, expression of chrASO in S. oneidensis and E. coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction.
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