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A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci
Published on: October 29, 2018
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Updated mtCOI reference dataset for the Bemisia tabaci species complex.
Laura M Boykin1, Anders Savill1, Paul De Barro2
1School of Molecular Sciences and Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Perth, WA, Australia.
F1000Research
|November 24, 2017
Summary
An updated reference dataset of Bemisia tabaci mitochondrial COI gene sequences is now available. This resource aids in identifying this invasive whitefly species, which causes significant agricultural damage globally.
Area of Science:
- Entomology
- Molecular Biology
- Genetics
Background:
- The whitefly Bemisia tabaci species complex is a globally invasive pest causing substantial agricultural losses.
- Bemisia tabaci species are morphologically indistinguishable, necessitating molecular methods for identification.
- Existing reference datasets for Bemisia tabaci identification are outdated.
Purpose of the Study:
- To create an updated and comprehensive reference dataset of Bemisia tabaci mitochondrial cytochrome oxidase subunit I (mtCOI) gene sequences.
- To improve the accuracy and reliability of Bemisia tabaci species identification.
Main Methods:
- Downloaded all available Bemisia tabaci mtCOI gene sequences from GenBank on May 22, 2017.
- Performed quality checking on the downloaded sequences.
- Generated a curated dataset of 1,071 unique sequences, each 696 base pairs long.
Main Results:
- A new, high-quality dataset of 1,071 unique Bemisia tabaci mtCOI sequences was generated.
- The dataset includes sequences from 2013-2017, addressing the gap in the previous reference set.
- The dataset is publicly available for use in species identification.
Conclusions:
- The newly generated dataset provides a crucial update for identifying Bemisia tabaci species.
- This resource will aid researchers and agricultural professionals in managing this invasive pest.
- Accurate species identification is vital for effective control strategies against Bemisia tabaci.
