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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Solvent Front Position Extraction procedure with thin-layer chromatography as a mode of multicomponent sample
A Klimek-Turek1, E Sikora1, T H Dzido1
1Department of Physical Chemistry, Medical University, Lublin, Poland.
Abstract:
A concept of using thin-layer chromatography to multicomponent sample preparation for quantitative determination of solutes followed by instrumental technique is presented. Thin-layer chromatography (TLC) is used to separate chosen substances and their internal standard from other components (matrix) and to form a single spot/zone containing them at the solvent front position. The location of the analytes and internal standard in the solvent front zone allows their easy extraction followed by quantitation by HPLC.
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