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Differential polyadenylation of the mutant vasopressin mRNA during development of Brattleboro rats

S Fehr1, H Schmale, D Richter

  • 1Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus, Eppendorf, Hamburg, FRG.

Biochemical and Biophysical Research Communications
|January 31, 1989
PubMed

Insights

Vasopressin mRNA expression begins earlier in normal rats than in Brattleboro rats. Mutant vasopressin transcripts show developmental changes in polyadenylation, impacting gene expression.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Vasopressin is a crucial neurohormone involved in regulating water balance and blood pressure.
  • The Brattleboro rat is a genetic model with a defective vasopressin gene, exhibiting diabetes insipidus.

Purpose of the Study:

  • To investigate the developmental expression patterns of vasopressin mRNA in normal and Brattleboro rats.
  • To analyze differences in vasopressin mRNA size and polyadenylation during development between the two rat strains.

Main Methods:

  • In situ hybridization of hypothalamic sections to detect mRNA.
  • Analysis of mRNA size and polyadenylation using RNase H and oligo(dT) treatment.

Main Results:

  • Vasopressin mRNA appears at embryonic day 15 in Wistar rats and not before fetal day 18 in Brattleboro rats.
  • Oxytocin gene expression begins at fetal day 19 in both strains.
  • Adult Brattleboro rats exhibit larger vasopressin transcripts due to extended poly(A) sequences, unlike normal rats.

Conclusions:

  • The mutant vasopressin gene transcript in Brattleboro rats undergoes cellular-specific differential polyadenylation during development.
  • This altered polyadenylation may contribute to the physiological differences observed in Brattleboro rats.

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