N₂O strongly prevents adhesion formation and postoperative pain in open surgery through a drug-like effect

Microsurgical tenets and peritoneal conditioning during laparoscopic surgery (LS) decrease postoperative adhesions and pain. For a trial in human, the strong beneficial effects of N₂O needed to be confirmed in open surgery (OS).

In a mouse model for OS, the effect of the gas environment upon adhesions was evaluated. Experiment I evaluated desiccation and the duration of exposure to CO₂, N₂O or CO₂ + 4%O₂. Experiment II evaluated the dose-response curve of adding N₂O to CO₂. Experiment III compared humidified CO₂ + 10% N₂O during LS and OS.In OS, 30- and 60-min exposure to non-humidified CO₂ caused mortality of 33 and 100%, respectively. Mortality was prevented by humidification, by dry N₂O or dry CO₂ + 4%O₂. Adhesions increased with the duration of exposure to CO₂ (p < 0.0001) and decreased slightly by humidification or by the addition of 4% O₂. N₂O strongly decreased adhesions at concentrations of 5% or greater. With humidified CO₂ + 10% N₂O, adhesion formation was similar in OS and LS.

The drug-like and strong beneficial effect of low concentrations of N₂O is confirmed in OS.