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Updated: Feb 18, 2026

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
Published on: December 23, 2016
[18F]ODIBO: a prosthetic group for bioorthogonal radiolabeling of macromolecules via strain-promoted alkyne-azide
Mehdi Boudjemeline1, Christopher D McNitt, Thomas A Singleton
1McConnell Brain Imaging Centre, Montreal Neurological Institute, McGill University, Montreal, Canada. alexey.kostikov@mcgill.ca.
Abstract:
A novel prosthetic group for the efficient radiolabeling of macromolecules has been developed. [18F]oxadibenzocyclooctyne ([18F]ODIBO) is synthesized in high radiochemical yield and applied for nearly quantitative conjugation to azide-tagged peptides and proteins at room temperature and low substrate concentrations. The resulting bioconjugates are chemically and radiochemically pure and free of toxic solvents and catalysts.
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