Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple preservative for flow cytometric DNA analysis.

K A Alanen1, P J Klemi, S Taimela

  • 1Department of Pathology, University of Turku, Finland.

Cytometry
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety, pharmacokinetics, pharmacodynamics, and antitumor activity of cergutuzumab amunaleukin: a phase I study in patients with advanced and/or metastatic solid tumors.

ESMO open·2026
Same author

Including tumor-infiltrating lymphocytes into the PREDICT prognostic model for triple-negative breast cancer survival.

Annals of oncology : official journal of the European Society for Medical Oncology·2026
Same author

Risk factors for shoulder pain and stiffness in adults aged 44 and older: an 11-year longitudinal population-based study.

Scandinavian journal of rheumatology·2024
Same author

Gastrointestinal stromal tumours: ESMO-EURACAN-GENTURIS Clinical Practice Guidelines for diagnosis, treatment and follow-up.

Annals of oncology : official journal of the European Society for Medical Oncology·2021
Same author

Bone sarcomas: ESMO-EURACAN-GENTURIS-ERN PaedCan Clinical Practice Guideline for diagnosis, treatment and follow-up.

Annals of oncology : official journal of the European Society for Medical Oncology·2021
Same author

Soft tissue and visceral sarcomas: ESMO-EURACAN-GENTURIS Clinical Practice Guidelines for diagnosis, treatment and follow-up<sup>☆</sup>.

Annals of oncology : official journal of the European Society for Medical Oncology·2021
Same journal

Measurement of the distribution of red blood cell deformability using an automated rheoscope.

Cytometry·2002
Same journal

Flow cytometric-based isolation of nucleated erythroid cells during maturation: an approach to cell surface antigen studies.

Cytometry·2002
Same journal

Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients.

Cytometry·2002
Same journal

Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.

Cytometry·2002
Same journal

Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

Cytometry·2002
Same journal

Separation of live cells in different phases of the cell cycle for gene expression analysis.

Cytometry·2002
See all related articles

A new cell preservation method using citric acid buffered saline (CABS) and ethanol (E) offers better DNA analysis results. This CABS+E solution improves sample quality for flow cytometry, reducing insufficient samples and enhancing DNA content resolution.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Medical Diagnostics

Background:

  • Accurate DNA content analysis is crucial for diagnosing various diseases.
  • Traditional cell preservation methods can lead to suboptimal sample quality and reduced analytical resolution.
  • Flow cytometry is a key technique for DNA analysis, requiring well-preserved cell samples.

Purpose of the Study:

  • To evaluate a novel cell preservation solution composed of citric acid buffered saline (CABS) and 99% ethanol (E) in a 1:1 ratio.
  • To compare the efficacy of CABS+E preservation with CABS alone and 50% ethanol for flow cytometric DNA analysis.
  • To determine the optimal preservation method for cytological samples intended for DNA content analysis.

Main Methods:

  • Fine needle biopsy aspirates were preserved using three different solutions: CABS+E (1:1), CABS alone, and 50% ethanol.

Related Experiment Videos

  • DNA histograms were generated from preserved samples using flow cytometry.
  • Mean coefficient of variation (CV) and the number of cells per sample were analyzed and compared across preservation methods.

    • Main Results:

    • The CABS+E solution yielded DNA histograms with a mean CV (3.3%) comparable to CABS alone (3.4%).
    • CABS+E preservation resulted in significantly lower mean CV (P < .0001) compared to 50% ethanol (mean 4.8%).
    • Samples preserved in CABS+E and 50% ethanol had significantly fewer insufficient samples (low cell count) compared to CABS alone (P < .0001).

    Conclusions:

    • Preservation of cells in CABS+E allows for long-term storage of samples.
    • The CABS+E method reduces the number of insufficient samples compared to buffered saline alone.
    • CABS+E offers enhanced DNA content resolution compared to 50% ethanol, making it recommended for cytological sample preservation for flow cytometry.