Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

8.7K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.7K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

17.0K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
17.0K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

13.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
13.2K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.1K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.1K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.8K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of Antimicrobial Susceptibility Patterns of Bacterial Isolates from Blood, Urine, and Lower Respiratory Tract Specimens Between Elderly Patients in Long-Term Care Hospitals and Community-Acquired Infections: A Retrospective Study.

Antibiotics (Basel, Switzerland)·2026
Same author

Structure-Guided Identification of Phytochemical OCT2 Inhibitors and Their Functional Relevance to Cisplatin-Induced Cytotoxicity.

Pharmaceutics·2026
Same author

Supercritically Extracted Mealworm Oil Alleviates High-Fat Diet-Induced Obesity by Modulating Gut Microbiota in Mice.

Molecular nutrition & food research·2026
Same author

Vertically Stacked Paper-Based Microarray Device for High-Throughput SERS Detection of Two Cancer Biomarkers.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Polystyrene microplastics induce skeletal muscle atrophy through disruption of anabolic signaling and mitochondrial function.

Toxicology·2026
Same author

Feasibility and Preliminary Effectiveness of a Hybrid mHealth Intervention for People with Disabilities: A Pilot Study.

Inquiry : a journal of medical care organization, provision and financing·2026

Related Experiment Video

Updated: Feb 18, 2026

Examining BCL-2 Family Function with Large Unilamellar Vesicles
08:35

Examining BCL-2 Family Function with Large Unilamellar Vesicles

Published on: October 5, 2012

8.4K

Structural basis for the interaction between DJ-1 and Bcl-XL.

Mi-Kyung Lee1, Min-Sung Lee2, Da-Woon Bae3

  • 1Disease Target Structure Research Center, KRIBB, Daejeon 34141, Republic of Korea.

Biochemical and Biophysical Research Communications
|November 28, 2017
PubMed
Summary

Oxidized DJ-1 protein binds to the Bcl-XL protein in mitochondria, protecting cells from death. This interaction, particularly via DJ-1's C-terminal peptide, offers a structural basis for DJ-1's role in cell survival pathways.

Keywords:
Bcl-X(L)Complex structureDJ-1NMR spectroscopyProtein interaction

More Related Videos

High Content Screening in Neurodegenerative Diseases
13:32

High Content Screening in Neurodegenerative Diseases

Published on: January 6, 2012

18.2K
Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

18.6K

Related Experiment Videos

Last Updated: Feb 18, 2026

Examining BCL-2 Family Function with Large Unilamellar Vesicles
08:35

Examining BCL-2 Family Function with Large Unilamellar Vesicles

Published on: October 5, 2012

8.4K
High Content Screening in Neurodegenerative Diseases
13:32

High Content Screening in Neurodegenerative Diseases

Published on: January 6, 2012

18.2K
Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

18.6K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • DJ-1 is a multifunctional protein implicated in Parkinson's disease and cancer.
  • DJ-1 translocates to mitochondria upon UVB irradiation, interacting with Bcl-XL to prevent cell death.

Purpose of the Study:

  • To structurally characterize the molecular interaction between DJ-1 and Bcl-XL.
  • To elucidate the binding mechanism of DJ-1 to Bcl-XL.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy, specifically chemical shift perturbation.
  • Structural modeling of protein complexes.

Main Results:

  • NMR data revealed that oxidized DJ-1 binds to a hydrophobic groove on Bcl-XL.
  • The C-terminal α8-helix peptide (Cpep) of DJ-1 functions as a Bcl-XL-binding motif.
  • The binding mode of DJ-1 Cpep to Bcl-XL resembles that of other pro-apoptotic BH3 peptides.

Conclusions:

  • The study provides a structural foundation for understanding how DJ-1 interacts with Bcl-XL.
  • This interaction is crucial for regulating Bcl-XL activity under oxidative stress conditions.
  • Findings contribute to the molecular understanding of DJ-1's role in cell survival and disease pathogenesis.