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Microbial volatile communication in human organotypic lung models.

Layla J Barkal1,2, Clare L Procknow3, Yasmín R Álvarez-García4

  • 1Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA.

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|November 28, 2017
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Summary

Researchers developed a novel microscale human bronchiole model to study lung infections. This advanced model allows for the investigation of host-pathogen interactions and volatile compound communication in pulmonary disease.

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Area of Science:

  • Pulmonary Medicine
  • Microbiology
  • Biotechnology

Background:

  • Inhaling respiratory pathogens triggers complex host-microbe signaling, leading to varied outcomes like clearance, colonization, or disease.
  • Traditional in vitro methods lack the complexity to accurately model the lung microenvironment for studying these interactions.
  • Understanding these early signaling events is crucial for developing effective treatments for pulmonary infections.

Purpose of the Study:

  • To introduce a novel microscale organotypic model of the human bronchiole for studying pulmonary infections.
  • To enable the investigation of host-pathogen signaling within a physiologically relevant lung microenvironment.
  • To explore volatile compound-mediated communication between microbes and the lung model.

Main Methods:

  • Development of a microscale organotypic model mimicking human bronchiole structure, including airway, vascular, and extracellular matrix compartments.
  • Integration of a clickable extension to facilitate volatile compound exchange between microbial cultures and the lung model.
  • Infection of the model with Aspergillus fumigatus to characterize the inflammatory response.

Main Results:

  • The organotypic bronchiole model successfully replicated key structural features of the human airway.
  • Characterization of the inflammatory response of the model to Aspergillus fumigatus infection was achieved.
  • Demonstration of multikingdom, volatile-mediated communication between the lung model and co-cultured Aspergillus fumigatus and Pseudomonas aeruginosa.

Conclusions:

  • The microscale organotypic bronchiole model provides a powerful platform for studying pulmonary infections.
  • This model facilitates the investigation of complex host-pathogen interactions and microbial communication in the lung.
  • The findings open new avenues for understanding and potentially treating respiratory diseases caused by microbial pathogens.