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Rapid re-identification of human samples using portable DNA sequencing
Sophie Zaaijer1,2, Assaf Gordon2, Daniel Speyer1,2
1Department of Computer Science, New York Genome Center, New York, United States.
Elife
|November 29, 2017
Summary
MinION sketching offers rapid, inexpensive DNA re-identification using few single nucleotide polymorphisms (SNPs). This method enables near real-time applications for cell line and tissue authentication, improving research reproducibility.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- DNA re-identification is crucial for applications like cell line authentication and forensics.
- Current DNA re-identification methods face challenges with high latency and restricted access.
Purpose of the Study:
- To introduce 'MinION sketching,' a novel, rapid, and portable strategy for human DNA re-identification.
- To enable near real-time DNA re-identification applications.
Main Methods:
- Utilizes the MinION sequencing platform for rapid DNA analysis.
- Requires minimal sequencing time (as few as 3 minutes) and a small number of random single nucleotide polymorphisms (SNPs) (60-300).
- Leverages the increasing availability of genomic reference data.
Main Results:
- Achieves robust human DNA re-identification with high speed and low cost.
- Demonstrates the potential for near real-time DNA re-identification.
- Enables efficient cell line and tissue authentication in research and clinical settings.
Conclusions:
- MinION sketching provides a significantly faster and more reliable alternative to current cell line authentication practices.
- This method can help mitigate research irreproducibility issues stemming from cell culture mix-ups and contamination.
- Facilitates periodic authentication of cell lines and tissues in biobanks and research.
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