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A colorimetric competitive displacement assay for the evaluation of catalytic peptides
Anneliese M M Gest1, Erika M Aguiluz, Malik T Mays
1Department of Chemistry, Macalester College, 1600 Grand Ave., Saint Paul, MN 55105, USA. lwitus@macalester.edu.
Organic & Biomolecular Chemistry
|November 29, 2017
Abstract:
An indicator displacement assay has been adapted to detect the diol products of the aldol reaction between 4-nitrobenzaldehyde and hydroxyacetone in crude reaction mixtures. This provides a rapid colorimetric method of detecting product formation and thus evaluating potential catalysts, which is demonstrated using multiple catalytic peptides.

