Related Experiment Video
Updated: Feb 17, 2026
Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
Published on: February 1, 2022
Universal fluorometric aptasensor platform based on water-soluble conjugated polymers/graphene oxide.
Limin Guo1, You Hu1, Ziqi Zhang1
1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, No. 620, West Chang'an Avenue Chang'an District, Xi'an, Shaanxi, 710062, China.
This study introduces a universal aptasensor for detecting pollutants and biomolecules using fluorescence resonance energy transfer (FRET). The novel sensor demonstrates high sensitivity and selectivity for targets like Bisphenol A and dopamine in real samples.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Materials Science
Background:
- Fluorescence resonance energy transfer (FRET) is a powerful tool for molecular sensing.
- Graphene oxide (GO) offers unique quenching properties for biosensor development.
- Aptasensors provide high specificity and sensitivity for target detection.
Purpose of the Study:
- To develop a universal and sensitive fluorometric aptasensor.
- To utilize a FRET mechanism for target detection.
- To validate the aptasensor's performance for pollutant and biomolecule detection.
Main Methods:
- Designed a FRET-based aptasensor using poly(9,9-bis(6'-N,N,N-trimethylammonium)hexyl)fluorine phenylene (PFP) as donor and FAM-labeled aptamer as acceptor.
- Employed graphene oxide (GO) as a quencher, which adsorbs aptamers in the absence of targets.
- Validated the aptasensor with Bisphenol A (BPA) and dopamine, and demonstrated universality with adenosine detection.
Main Results:
- Achieved sensitive detection of BPA (LOD 0.005 ng/mL) and dopamine (LOD 1.0 nmol/L).
- Demonstrated high selectivity and sensitivity, with good recovery rates in human plasma and serum.
- Confirmed the aptasensor's universality by successfully detecting adenosine, proving adaptability for other analytes.
Conclusions:
- The developed aptasensor is universal, sensitive, and selective for detecting small molecules.
- The FRET-based mechanism with GO offers a robust platform for various analytes.
- This aptasensor has potential applications in environmental monitoring and clinical diagnostics.
