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Plasma EBV microRNAs in paediatric renal transplant recipients

Jaythoon Hassan1, Jonathan Dean2, Cillian F De Gascun2

  • 1National Virus Reference Laboratory, University College Dublin, Belfield, Dublin 4, Ireland. jaythoon.hassan@ucd.ie.

Journal of Nephrology
|November 30, 2017
PubMed
Abstract

Insights

Epstein-Barr virus (EBV) microRNAs (miRNAs) were profiled in pediatric kidney transplant patients. EBV-miR-BART2-5p may sustain high viral loads in chronic high EBV patients, potentially aiding in post-transplant lymphoproliferative disease development.

Area of Science:

  • Virology
  • Immunology
  • Transplantation

Background:

  • Epstein-Barr virus (EBV) was the first human virus found to express microRNAs (miRNAs).
  • 44 mature miRNAs are encoded in the EBV genome.
  • EBV miRNA profiles in pediatric renal transplant recipients have not been previously studied.

Purpose of the Study:

  • To investigate circulating EBV miRNA profiles as potential biomarkers in pediatric renal transplant patients.
  • To explore the role of EBV miRNAs in different EBV infection states post-transplantation.

Main Methods:

  • Examined 42 EBV miRNAs from BART and BHRF open reading frames.
  • Analyzed samples from renal transplant recipients with resolved EBV infection (REI) or chronic high viral loads (CHL).
  • Included non-transplant patients with acute infectious mononucleosis (IM) as controls.

Main Results:

  • Plasma EBV-miR-BART2-5p was elevated in IM and CHL patients compared to REI patients.
  • A trend suggested a correlation between EBV miRNA levels and EBV viral load.
  • Specific EBV-miRs were detected only in IM and CHL patients, while lytic EBV-miRs were found only in IM patients.
  • One CHL patient who developed post-transplant lymphoproliferative disease (PTLD) showed significantly higher EBV-miR-BART2-5p levels.

Conclusions:

  • EBV-miR-BART2-5p may contribute to maintaining high EBV viral loads in CHL pediatric kidney transplant recipients.
  • This miRNA targets MICB to evade NK cell recognition, potentially playing a role in PTLD development.
  • Circulating EBV miRNAs show promise as biomarkers in pediatric renal transplant recipients.
Keywords:
EBV miRNAEBV viral loadPlasma EBV-miR-BART2-5pRenal transplant recipients

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