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Related Experiment Video

Updated: Feb 17, 2026

Evaluation of Capillary and Other Vessel Contribution to Macular Perfusion Density Measured with Optical Coherence Tomography Angiography
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Pupil Dilation May Affect Retinal Vessel Caliber Measures.

Jie Jin Wang1,2, Yuan Shi2,3, Jing Xie4

  • 1a Centre for Vision Research, Department of Ophthalmology and Westmead Institute for Medical Research , University of Sydney, Sydney , Australia.

Ophthalmic Epidemiology
|November 30, 2017
PubMed
Summary
This summary is machine-generated.

Pupil dilation significantly impacts retinal vessel caliber measurements, affecting both arteriolar and venular equivalents. This finding is crucial for accurate retinal imaging analysis.

Keywords:
Before and after comparisonpupil dilationretinaretinal imagevessel caliber

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Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Retinal Imaging Analysis

Background:

  • Accurate measurement of retinal vessel caliber is essential for diagnosing and monitoring various ocular and systemic diseases.
  • Pupil dilation is a common procedure in ophthalmology, but its effect on quantitative retinal imaging metrics is not fully understood.

Purpose of the Study:

  • To investigate the influence of pupil dilation on the measurement of retinal arteriolar caliber equivalent (CRAE) and retinal venular caliber equivalent (CRVE).

Main Methods:

  • An observational study involving 25 subjects where retinal photographs were captured before and after pupil dilation.
  • Retinal images were analyzed by three masked graders using a computer-assisted method to measure CRAE and CRVE.
  • Statistical analyses included concordance correlation coefficients, Bland Altman limits-of-agreement, and linear mixed models.

Main Results:

  • High concordance (CCs 0.82-0.94) was observed between pre- and post-dilation images.
  • Mean differences in caliber measurements ranged from 0.55-3.42μm for CRAE and 1.56-2.29μm for CRVE.
  • A significant fixed effect of pupil dilation on mean CRAE was found in two of three graders, and on both CRAE and CRVE variances in the overall model.

Conclusions:

  • Contrary to the initial hypothesis, pupil dilation exerts a systematic effect on retinal vessel caliber measurements.
  • These findings highlight the importance of considering pupil dilation status in the interpretation of retinal vessel caliber data.