Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Widespread omission of aptamer-target binding verification in aptasensor development: Consequences for sensor performance and the need for a K<sub>d</sub> gate.

Biosensors & bioelectronics·2026

Same author

Assembly-Energetics-Control (AEC) Design Framework for Rotary DNA Nanomachines.

Chembiochem : a European journal of chemical biology·2026

Same author

Nanoplasmonic optical fiber sensing of SARS-CoV-2 nucleocapsid protein using an aptamer-DNA tetrahedron interface.

Journal of nanobiotechnology·2026

Same author

Acoustically seeded fabrication of a DNA tesseract into a conductive wire.

Nucleic acids research·2025

Same author

Designing the Future of Biosensing: Advances in Aptamer Discovery, Computational Modeling, and Diagnostic Applications.

Biosensors·2025

Same author

Probing three-dimensional cyclooctatetraene for nucleobase modification in aptamer selection.

Communications chemistry·2025

Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026

Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026

Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026

Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026

Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026

Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 17, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Aptamer Bioinformatics.

Andrew B Kinghorn¹, Lewis A Fraser¹, Shaolin Lang¹

¹School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR China

International Journal of Molecular Sciences

|December 1, 2017

Summary

This summary is machine-generated.

Bioinformatics enhances aptamer development by simulating selection, designing aptamer libraries, and optimizing sequences. These computational methods improve the efficiency and effectiveness of aptamer discovery and characterization.

Keywords:

HTS aptamer fragment based design in silico selection molecular dynamics simulation

More Related Videos

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Related Experiment Videos

Last Updated: Feb 17, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Area of Science:

Biochemistry
Bioinformatics
Molecular Biology

Background:

Aptamers are specific, high-affinity nucleic acid binders.
Systematic Evolution of Ligands by Exponential Enrichment (SELEX) is the standard isolation method.
Aptamers' genetic nature allows for computational improvements.

Purpose of the Study:

To review advancements in aptamer bioinformatics.
To highlight computational approaches for aptamer selection and design.

Main Methods:

Simulation of aptamer selection processes.
Fragment-based aptamer design strategies.
Library patterning and high-throughput sequencing (HTS) data analysis.
In silico aptamer optimization techniques.

Main Results:

Bioinformatics significantly refines aptamer selection efficiency.
Computational tools enable novel aptamer library designs.
HTS data analysis accelerates lead aptamer identification.
In silico optimization enhances aptamer performance.

Conclusions:

Bioinformatics is crucial for advancing aptamer technology.
Computational approaches streamline aptamer discovery and development.
Future aptamer research will increasingly rely on bioinformatics tools.